29 PHT was induced by a black and white vans portal vein constriction as previously described. 7 A laparotomy was performed under halothane anesthesia. The portal vein was isolated, and calibrated constriction was performed using a single ligature of 3-0 silk around the portal vein and a 27-gauge blunt-tipped needle. The needle was then removed, leaving a calibrated stenosis of the portal vein. This technique involved the initial constriction of the portal vein to <"50% of its diameter. In SO mice, the same operation was performed with the exception that no ligature was placed after isolating the portal vein.
Appropriate horseradish peroxidase-conjugated secondary antibodies were used to detect the primary antibody/antigen complexes. The signal was detected using ECL Western blotting detection reagents (Amersham Pharmacia Biotech, Piscataway, NJ, USA). After quantifying the vans mens shoes signals by densitometry, the results are expressed as a ratio to loading control densitometry units.Total RNA was isolated from the gastric mucosal scraping samples using the RNAgents Total RNA Isolation System (Promega, Madison, WI, USA) according to the manufacturer's instructions. First-strand cDNA rainbow vans was synthesized using Superscript Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions.
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